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p smad2 3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p smad2 3
    P Smad2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p+smad2+3/Phospho-Smad2+(Ser465%2F467)%2FSmad3+(Ser423%2F425)+Rabbit+mAb/pm41776505-67-37-44
    Average 96 stars, based on 1239 article reviews
    p smad2 3 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis
    Article Snippet: After the total protein concentrations were detected using a bicinchoninic acid (BCA) protein assay (Beyotime, China), an equal amount of protein from each sample was resolved by SDS-PAGE on 10% SDS-polyacrylamide gels and transferred to a polyvinylidene difluoride (PVDF) membrane for immunoblot analyses. .. The membranes were incubated with the following primary antibodies overnight at 4°C: rabbit anti-GDF11 (1:1000; Abcam, ab124721, United States), rabbit anti-Bax (1:1000; affinity Biosciences, AF0120, United States), rabbit anti-caspase3 (1:1000; affinity Biosciences, DF6879, United States), rabbit anti-Bcl2 (1:500, Proteintech, 12789-1-AP, United States), rabbit anti-smad2/3 (1:1000, CST, 8685S,United States), rabbit anti-p-smad2/3 (1:1000, CST, 8828S, United States), rabbit anti-smad1/5/8 (1:1000, abcam, ab66737, United States), rabbit anti-p-smad1/5/8 (1:1000, CST, 13820S, United States), and mouse anti-β-actin (1:10000; CST, 8H10D10, United States). .. The unbound antibodies on the PVDF membranes were washed, the secondary antibody (1:10000, CST, 5470P, United States), and (1:10000, CST, 5366P, United States) was added, and the bands were visualized with an infrared laser imaging system (Li-COR, United States).

    Article Title: Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease.
    Article Snippet: .. The PVDF was incubated with several primary monoclonal antibodies: rabbit anti-Smad2/3 (CST, #8685, 1:1000), rabbit anti-p-Smad2/3 (CST, #8828, 1:1000), rabbit anti-JNK (CST, #9552, 1:1000), rabbit anti-pJNK (CST, #4668, 1:1000), rabbit anti-ERK1/2 (CST, #4695, 1:1000), rabbit anti-p-ERK1/2 (CST, #4370, 1:1000), rabbit antip38 (CST, #9212, 1:1000), rabbit anti-p-p38 (CST, #4631, 1:1000), rabbit anti-Tartrate Resistant Acid Phosphatase (Abcam, #ab191406, 1:2000), mouse anti-Smad4 (Santa, #sc7966, 1:1000), rabbit anti-NFATc1 (CST, #8032, 1:1000), rabbit anti-RhoA (CST, #2117, 1:1000), rabbit anti-Cdc42 (CST, #2466, 1:1000) and mouse anti-b-actin (Sigma, 1:10000). .. Rabbit polyclonal antibodies anti-Rac1/2/3 (CST, #2465, 1:1000) were also used.

    Article Title: Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease
    Article Snippet: .. The PVDF was incubated with several primary monoclonal antibodies: rabbit anti-Smad2/3(CST, #8685, 1:1000), rabbit anti-p-Smad2/3 (CST, #8828, 1:1000), rabbit anti-JNK (CST, #9552, 1:1000), rabbit anti-p-JNK (CST, #4668, 1:1000), rabbit anti-ERK1/2 (CST, #4695, 1:1000), rabbit anti-p-ERK1/2 (CST, #4370, 1:1000), rabbit anti-p38 (CST, #9212, 1:1000), rabbit anti-p-p38 (CST, #4631, 1:1000), rabbit anti-Tartrate Resistant Acid Phosphatase (Abcam, #ab191406, 1:2000), mouse anti-Smad4 (Santa, #sc-7966, 1:1000), rabbit anti-NFATc1 (CST, #8032, 1:1000), rabbit anti-RhoA (CST, #2117, 1:1000), rabbit anti-Cdc42 (CST, #2466, 1:1000) and mouse anti-β-actin (Sigma, 1:10000). .. Rabbit polyclonal antibodies anti-Rac1/2/3 (CST, #2465, 1:1000) were also used.

    Bioprocessing:

    Article Title: Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease.
    Article Snippet: .. The PVDF was incubated with several primary monoclonal antibodies: rabbit anti-Smad2/3 (CST, #8685, 1:1000), rabbit anti-p-Smad2/3 (CST, #8828, 1:1000), rabbit anti-JNK (CST, #9552, 1:1000), rabbit anti-pJNK (CST, #4668, 1:1000), rabbit anti-ERK1/2 (CST, #4695, 1:1000), rabbit anti-p-ERK1/2 (CST, #4370, 1:1000), rabbit antip38 (CST, #9212, 1:1000), rabbit anti-p-p38 (CST, #4631, 1:1000), rabbit anti-Tartrate Resistant Acid Phosphatase (Abcam, #ab191406, 1:2000), mouse anti-Smad4 (Santa, #sc7966, 1:1000), rabbit anti-NFATc1 (CST, #8032, 1:1000), rabbit anti-RhoA (CST, #2117, 1:1000), rabbit anti-Cdc42 (CST, #2466, 1:1000) and mouse anti-b-actin (Sigma, 1:10000). .. Rabbit polyclonal antibodies anti-Rac1/2/3 (CST, #2465, 1:1000) were also used.

    Article Title: Aberrant activation of TGF-β1 induces high bone turnover via Rho GTPases-mediated cytoskeletal remodeling in Camurati-Engelmann disease
    Article Snippet: .. The PVDF was incubated with several primary monoclonal antibodies: rabbit anti-Smad2/3(CST, #8685, 1:1000), rabbit anti-p-Smad2/3 (CST, #8828, 1:1000), rabbit anti-JNK (CST, #9552, 1:1000), rabbit anti-p-JNK (CST, #4668, 1:1000), rabbit anti-ERK1/2 (CST, #4695, 1:1000), rabbit anti-p-ERK1/2 (CST, #4370, 1:1000), rabbit anti-p38 (CST, #9212, 1:1000), rabbit anti-p-p38 (CST, #4631, 1:1000), rabbit anti-Tartrate Resistant Acid Phosphatase (Abcam, #ab191406, 1:2000), mouse anti-Smad4 (Santa, #sc-7966, 1:1000), rabbit anti-NFATc1 (CST, #8032, 1:1000), rabbit anti-RhoA (CST, #2117, 1:1000), rabbit anti-Cdc42 (CST, #2466, 1:1000) and mouse anti-β-actin (Sigma, 1:10000). .. Rabbit polyclonal antibodies anti-Rac1/2/3 (CST, #2465, 1:1000) were also used.

    other:

    Article Title: Increased activity of mesenchymal ALK2-BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues
    Article Snippet: Rabbit anti p-Smad2/3 , #3101, Cell signaling, Danvers, MA , 1:1,000.



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